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Phage gDNA extraction (Qiagen DNEasy kit)
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Lab Protocols
Phage Gdna Extraction (Qiagen Dneasy Kit)
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Commit
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2026-05-04 19:18:59
Bhaskar Kumawat
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lab protocols/phage gdna extraction (qiagen dneasy kit).md
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# Phage gDNA extraction (Qiagen DNEasy kit)
## Materials required
-
- Phage Lysate: 500uL high titer (~$10^{10}$), filtered with 0.22uM filters
+
- Phage Lysate: 500uL high titer (~ $10^{9}-10^{10}$ PFU/mL), filtered with 0.22uM filters
- DNAse I: 1U total
- RNAse A: 1uL of 10mg/mL (0.01 mg total)
- DNAse buffer: 50uL of 10X
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