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Commit 6f9480

2026-05-04 19:20:05 Bhaskar Kumawat: -/-
lab protocols/phage gdna extraction (qiagen dneasy kit).md ..
@@ 2,15 2,15 @@
## Materials required
- Phage Lysate: 500uL high titer (~ $10^{9}-10^{10}$ PFU/mL), filtered with 0.22uM filters
- - DNAse I: 1U total
- - RNAse A: 1uL of 10mg/mL (0.01 mg total)
+ - DNAse I: 0.5uL of 2U/uL (1 U total)
+ - RNAse A: 0.5uL of 20mg/mL (0.01 mg total)
- DNAse buffer: 50uL of 10X
- EDTA: 20uL of 0.5M
- Proteinase K: 1.25 uL of 20mg/mL
- DNAeasy Blood & Tissue Kit (Buffers AL, AW1, AW2, AE)
- - DNA Wash Buffer: 80% EtOH + Tris-HCL
+ - DNA Wash Buffer: 80% EtOH + 10mM Tris-HCl @ pH 7.5
- 100% EtOH
- - Nuclease free H2O
+ - Nuclease free H2O for elution
## Procedure
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