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2026-04-29 14:21:46 Bhaskar Kumawat: partial protocol

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`phage gdna extraction (pci).md` ..
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	# Phage gDNA extraction (PCI)
+
+	## Materials required:
+
+	### For phage lysis
+	- Phage lysate: 500uL high titer, filtered with 0.22uM filters
+	- DNAse I: 10U total
+	- RNAse A: 1uL of 100mg/mL (0.1 mg total)
+	- DNAse buffer: 50uL of 10X
+	- EDTA: 20uL of 0.5M
+	- Proteinase K: 2.5U total
+	- SDS: 25uL of 10%
+
+	### For DNA extraction
+	- TE-saturated Phenol:Choloroform:Isoamyl alcohol 25:24:1
+	- Chloroform:Isoamyl alcohol 24:1
+
+
+
+	## Sources
+
+	[https://www.pnas.org/doi/10.1073/pnas.2104592118#sec-3](https://www.pnas.org/doi/10.1073/pnas.2104592118#sec-3)
+	[At the Bench: A Laboratory Navigator (pg 284, pg 145)](https://www.cshlpress.com/default.tpl?cart=1777472107764588063&fromlink=T&linkaction=full&linksortby=oop_title&--eqSKUdatarq=470)